Green synthesis
of Silver Nanoparticles using Premna tomentosa leaf extract and its effective antibacterial
activity
Hebron Kirubai Raj S.D. and Danie Kingsley J.
Res. J. Chem. Environ; Vol. 26(12); 85-91;
doi: https://doi.org/10.25303/2612rjce085091; (2022)
Abstract
Green synthesis of silver nanoparticles was done at room temperature using Premna
tomentosa leaf extract. Silver nanoparticle formation was preliminarily confirmed
through UV Spectroscopy and characterized by FTIR, XRD, TEM and SEM. The λmax was
observed at 424nm in UV spectroscopy results. The XRD and EDAX spectrum confirmed
the presence of silver ions and the crystalline nature of synthesized silver nanoparticles.
The synthesized silver nanoparticles were spherical, hexagonal and irregular in
shape. FTIR results have revealed the functional groups present in the synthesized
nanoparticles such as C-N stretch, N-O symmetric stretch, C-H stretch, N-H bend,
C-H group, O-H stretch and H bond.
TEM and SEM results showed a clear image of nanoparticles in the size range 100nm
and 200nm. In the application part, we focused on the antibacterial activity of
synthesized nanoparticles on six pathogens namely Escherichia coli, Bacillus subtilis,
Staphylococcus aureus, Pseudomonas aeruginosa, Vibrio parahaemolyticus and Proteus
mirabilis. The maximum zone of inhibition was found in Vibrio parahaemolyticus followed
by Staphylococcus aureus and Pseudomonas aeruginosa.
