A Sensitive and
High Throughput LC-MS/MS Method for the Determination of Resveratrol in Peanut Matrix
Pasupuleti Vidya Sagar Venkata, Bagade Prashant and Kachireddy Naga Suresh Reddy
Venkata
Res. J. Chem. Environ; Vol. 27(9); 46-52;
doi: https://doi.org/10.25303/2709rjce046052; (2023)
Abstract
A precise and very efficient tandem mass spectrometry method (LC-MS/MS) was developed
to quantify the resveratrol in the peanut matrix. Two mobile phases made up of water
and acetonitrile in a ratio of 98:2 (%v/v) with 0.1% formic acid and acetonitrile
and water in a percentage of 98:2 %(v/v) with 0.1% formic acid were used in the
LC-MS/MS analysis of resveratrol on a BEH C-18, 130A° (50mm 2.1mm, 1.7μm) analytical
column with 0.3 mL/min of flow rate.
A triple quadrupole mass spectrometer and a positive electrospray ionization system
were used to quantify resveratrol in multiple reaction monitoring (MRM) modes. The
technique was evaluated and was found capable of quantifying resveratrol in peanut
samples starting at 10 ng/g in compliance with SANTE guidelines.
