Phytoligands analysis
of aqueous seed extract of Momordica charantia Linn. via GC-MS for its antidiabetic
activity
Bala Manju, Dhiman Anju, Garg Munish and Dureja Harish
Res. J. Chem. Environ; Vol. 27(9); 75-82;
doi: https://doi.org/10.25303/2709rjce075082; (2023)
Abstract
In India, Momordica charantia Linn. is utilized in juices, health supplements and
colouring agents. The aim of the current study is to analyze phytoligands by utilizing
gas chromatography mass spectroscopy of aqueous seed extract of Momordica charantia
Linn (MCA). The MCA was prepared by macerating the powdered seeds for 72 hrs followed
by filtration and lyophilization followed by an in vitro α-glucosidase inhibitory
activity. MCA was the potent inhibitor of α-glucosidase with IC50 value 73.75± 0.05
μg/ml. The analysis through GC-MS investigated 60 phytoconstituents which were validated
using Schrodinger suite version 2020-3 against α-glucosidase (PDB ID- 5NN5).
As per docking results, the existing phytochemicals azetidin-2-one 3,3-dimethyl-4-(1-aminoethyl)-,
alpha-l-rhamnopyranose, piperidine, 3-methyl-, 1,4-Dioxane-2,5-dione,3,6-dimethyl
and citronellol epoxide (R or S) from MCA, got the highest d-score of -7.230, -5.918,
-5.643, -5.312 and -5.185 kcal/mol and could be promising antidiabetic drug candidates.
