Differential ChIP-Seq
Analysis of O-GlcNAc and Adriamycin bound Chromatin Loci in Human Breast Cancer
Cells
Nigam M. and Awasthi G.
Res. J. Biotech.; Vol. 20(4); 129-136;
doi: https://doi.org/10.25303/204rjbt1290136; (2025)
Abstract
GlcNAcylation is a dynamic post-translational modification that is involved in human
diseases and a wide range of biological processes. There is evidence that GlcNAcylation
modifies some tumor-associated proteins, but in tumor progression, the role of GlcNAcylation
remains unclear. The decrease in cell surface E-cadherin is the molecular mechanism
underlying GlcNAcylation-induced breast cancer metastasis. p120 and β-catenin, but
not E-cadherin, are GlcNAcylated; the GlcNAcylation of p120 and β- catenin might
play roles in the decrease of cell surface E-cadherin. Moreover, immunohistochemistry
analysis indicated that the global GlcNAcylation level in breast tumor tissues is
elevated significantly as compared to corresponding adjacent tissues; further, GlcNAcylation
was significantly enhanced in metastatic lymph nodes compared to their corresponding
primary tumor tissues.
This study shows that GlcNAcylation enhances the migration/invasion of tumor cells
through the analysis of O-GlcNAc protein-bound chromatin loci in human breast cancer
cells. This is an important step towards understanding the role of this protein
in inducing breast cancer. The main aim of this study is to examine the O-GlcNAc
protein-bound chromatin loci in the human breast cancer cells MCF-7 and MCF-7/ADR
through differential ChIP-Seq analysis. Also, the prediction and identification
of overall binding sites of MCF-7 and MCF-7/ADR cells were performed by the ChIP-seq
strategy.
