Investigating
the Synergistic Effect of Chamaecostus cuspidatus Leaf Extract and para-Coumaric
Acid on Streptozotocin-induced Diabetic Nephropathy in Wistar Rats
Menaka Priya Balaji and Devi Rajeswari V.
Res. J. Biotech.; Vol. 20(4); 111-117;
doi: https://doi.org/10.25303/204rjbt1110117; (2025)
Abstract
The research investigation aims to examine the impairment of endothelial function
in a Wistar rat model of insulin-resistant diabetes mellitus triggered by a high-fat
diet and administration of 40mg/kg streptozotocin. Chamaecostus cuspidatus leaf
extract, para-coumaric acid and a combination of para-coumaric acid and Chamaecostus
cuspidatus leaf extract were administered to diabetic animals. A standard control
group was administered 100mg/kg of metformin. Interestingly, the findings of this
investigation indicated that Chamaecostus cuspidatus extract treatment effectively
regulated glucose levels (P < 0.05), while para-coumaric acid reduced lipoprotein
levels (P < 0.0001), the combination showed effectively regulated hematological
parameters and increased the antioxidant efficacy (P < 0.0001).
Moreover, this combined treatment has also been observed to enhance antioxidant
enzymes of SOD, CAT, GSH and GPX activities and also prevent lipid peroxidation
and ROS levels in the kidney tissue. The histopathology results of the kidney renal
cells revealed that the administration of synergistic treatment led to a decrease
in inflammation of a lower grade as well as a recovered endothelium layer. The experiment
revealed that the synergy between Chamaecostus cuspidatus and para-coumaric acid
regulates biochemical characteristics and enhances antioxidants, thus suggesting
an effective treatment for diabetic nephropathy.
