Production, partial
purification and characterization of amylase from Paenibacillus lactis PEL6, an
endophytic bacteria from Mitrephora heyneana
Divyaa N., Senthil Kumar J., Sithara N.V. and Thangavelu A.U.
Res. J. Biotech.; Vol. 20(8); 101-107;
doi: https://doi.org/10.25303/208rjbt1010107; (2025)
Abstract
In recent years, researchers are keenly focusing on discovering antifungals, enzymes,
immune suppressants and anticancer compounds produced by endophytic microorganisms,
aiming to improve human well-being. The objective of this study is to produce, purify
and characterize amylase produced by Paenibacillus lactis PEL6, an endophyte isolated
from Mitrephora heyneana. The enzyme screening study in starch agar plate demonstrated
the activity of amylase produced by Paenibacillus lactis. In ammonium sulphate precipitation
study, the optimal saturation level for precipitating amylase was determined as
40-50%. Ammonium sulphate fractionation increased specific activity of amylase in
crude enzyme from 0.347 to 0.556 and increased the purity of amylase to 1.6-fold.
The study on the effect of pH and temperature on amylase revealed that the optimum
pH and temperature for the action of amylase on starch are pH 6.5 and 22°C respectively.
The combination of amylase with commercial detergent was more effective in removing
stains from fabrics than using the commercial detergent alone. In summary, this
study validates that Paenibacillus lactis can act as a good source of amylase enzyme,
which has great potential to be utilized in detergent industries.
