Exploring the
antioxidant and anticancer properties of Punica granatum L. peels ethanolic extract
on Human Cancer Cells
Vignesh P. and Nethaji S.
Res. J. Biotech.; Vol. 20(12); 123-129;
doi: https://doi.org/10.25303/2012rjbt1230129; (2025)
Abstract
Plant extracts can be used in combination with traditional chemotherapy or used
as alternative sources for adjuvant cancer therapy, as some of these extracts have
no adverse effects and activate the cells of the immune system. The present study
investigated the antioxidant and anticancer activity of PGPEE against HGT-1 cell
line. In the present study, 293.2 mg GAE/g DW of total phenol, 126.5 mg CAE/g DW
of total flavonoid and 117.6 μg C3gE/g DW of total anthocyanin were obtained in
the PGPEE. Punica granatum peel ethanol extracts were studied by various free radical
scavenging assays including DPPH, FRAP and reducing power assays. Among this, FRAP
scavenging activity (32.5 μM) showed the potential activity against the various
concentration of PGPEE extract.
The present study investigated inhibitory effects of dried and fresh PGPEE extracts
on the inhibitory activity of tyrosinase. The fresh PGPEE showed the better inhibition
in monophenolase (82.28±10.4b mg/ml) than diphonalse (79.34±6.96ab mg/ml). In the
anticancer activity, the minimum cell viability (27.29%) was observed in the 500μg/ml
concentration of the PGPEE extract followed by the 400 μg/ml concentration (32.45%).
In vivo antiangiogenic effect PGPEE was also tested using CAM assay as an in vivo
model at a dose of 72.4 μg/ml. The inhibitory activity for the PGPEE (65.50 ± 3.5%)
inhibitions was observed.
