Analysis of co-culture
of Streptomyces sp. VITGV156 with four different bacteria for enhancing secondary
metabolites production
Ramesh Pooja and John Godwin Christopher
Res. J. Biotech.; Vol. 20(2); 173-182;
doi: https://doi.org/10.25303/202rjbt1730182; (2025)
Abstract
Streptomyces plays a pivotal role in producing antibiotics, essential for treating
diseases and enhancing human health. In this study, the endophytic novel Streptomyces
sp. VITGV156 was used to create four different consortia with live bacteria Staphylococcus
aureus, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa to activate
the production of secondary metabolites. The secondary metabolites produced in the
medium were extracted with ethyl acetate. The antimicrobial activity of the extracts
from the monocultures (VITGV156) and cocultures was tested. The zone of inhibition
for the monoculture crude extracts ranged from 10mm-20mm. The antimicrobial activity
of VITGV156 with E. coli ranged from 11mm-22mm and of VITGV156 with B. subtitles
was 10mm-20mm. The coculture of VITGV156 with S. aureus yielded a zone of 13mm-21mm.
Coculture of VITGV156 with P. aeruginosa showed a zone ranging from 12mm-21mm at
a concentration of 100mg/ml.
Crude ethylacetate extracts were investigated for their antioxidant activity which
was 77.67±0.2% for 156PA coculture followed by the other extracts. GC‒MS analysis
of these extracts revealed that five compounds were common in all four cocultures:
benzeneacetic acid, benzoic acid, 2-amino acid, pyrrolo[1,2-a] pyrazine-1,4-dione,
hexahydro-3-(2-methyl propyl), N-acetyltyramine and cyclo-(l-leucyl-l-phenylalanyl).
N-Acetyltyramine had a high percentage area. According to this study, all these
cocultures produce bioactive secondary metabolites.
