Apigenin in Apigenin
Nanoliposomes: Development and Validation of an Analytical Technique Based on HPTLC
Shetti P.P., Gudasi S. and Kubade M.
Res. J. Biotech.; Vol. 20(2); 13-19;
doi: https://doi.org/10.25303/202rjbt013019; (2025)
Abstract
Apigenin is a flavonoid found in various fruits and vegetables, aimed at its extensive
array of biological actions such as anti-oxidant, anti-viral, antibacterial, anti-inflammatory,
including chemo preventive property. Therefore, this study aimed to develop, describe
and evaluate apigenin nanoliposomes. In this study, ethanol injection method was
employed to formulate apigenin nanoliposomes. The quantification of apigenin in
the nanoliposomes was carried out using a HPTLC analysis technique. The defined
standards were followed in development and validation of the approach based on ICH
guidelines. An aluminium plate 60 F254 of silica gel was used as the stationary
phase, toluene, ethyl acetate and acetic acid ratio of 6:3:1 (v/v/v) was the mobile
phase.
Validation was done on the linearity, range, detection limit, quantification limit,
precision and robustness of the method. Reference standard for apigenin varied between
0.2-1 μg/band and a correlation value of 0.989 was observed. The results showed
that the LOQ was 0.29 μg /band and LOD was 0.095 μg /band. % RSD readings for intraday
and interday precision were determined to fall within the necessary range of 2 for
the apigenin standard. The established analytical method for assessing apigenin
in apigenin-loaded stealth liposomes has proven to be straightforward, reliable,
accurate and robust method.
