Callistemon viminalis
(Sol. ex Gaertn.) exhibiting anti-neuroblastoma activity via molecular interaction
of ascorbic acid and 5-lipoxygenase: An in vitro and in silico study
Rai Nitish, Sinha Saurabh K., Kumar Neeraj, Dey Sharmistha and Jain Vivek
Res. J. Biotech.; Vol. 20(2); 114-121;
doi: https://doi.org/10.25303/202rjbt1140121; (2025)
Abstract
Neuroblastoma is the extracranial of children characterized by diverse clinical
manifestations. The present study aimed to investigate the anti-neuroblastoma activity
of hydromethanolic extract of Callistemon viminalis (Sol. ex Gaertn.) flower. The
anti-neuroblastoma activity of hydromethanolic extract of C. viminalis flower was
analysed in SH-SY5Y cell line using MTT assay. The study also evaluated drug-likeness
and pharmacokinetic properties of bioactive compounds of extract using SwissADME
tool and conducted in silico analysis to determine the molecular interaction between
ascorbic acid and 5-LO for explaining the anti-neuroblastoma effect. Significant
cytotoxicity was observed in SH-SY5Y cells in a dose and time dependent manner.
The ADMET properties of the analysed bioactive compounds of C. viminalis showed
them as a suitable drug candidate with appropriate pharmacokinetic properties.
In silico results revealed that ascorbic acid binds into the catalytic domain and
interacted with Ser447, Tyr470 and Arg457 of 5-LO with a binding energy of -5.11
Kcal/mol. The total phenol and ascorbate content were found to be 18.71 mg CAE/gm
and 16.8 mg/100gm of extract, respectively. Hydromethanolic extract of C. viminalis
flower induced cytotoxic effect in human neuroblastoma SH-SY5Y cells via interaction
of its constituent ascorbic acid with the 5-LO, one of the important mediators of
Neuroblastoma.
