Exploring the
impact of Chlamydomonas reinhardtii Artificial Culture Medium on Ammonium and Phosphate
Removal in Aquaculture Wastewater
Nguyen My Phi-Long, Le Pham Dan-Khanh, Nguyen Tran Thuy Thanh-Mai, Nguyen Duc-Hung
and Nguyen Thi Gia-Hang
Res. J. Biotech.; Vol. 20(2); 33-42;
doi: https://doi.org/10.25303/202rjbt033042; (2025)
Abstract
This study utilized Chlamydomonas reinhardtii, an algal strain isolated from shrimp
aquaculture wastewater ponds in Ca Mau province to investigate its capacity for
ammonium and phosphate removal from wastewater. Cultivation occurred in both Tris-Phosphate-Acetate
(TAP) and Bold's Basal Medium (BBM) under controlled conditions: a temperature of
25 ± 1 0C and a 24-hour light cycle provided by light-emitting diodes (LEDs). Over
28 days, four different concentrations of ammonium and phosphate were tested.
Results demonstrated that algal biomass in the BBM medium exceeded threefold. Optimal
algal biomass was observed at approximately 1.0 mg N.L⁻¹ ammonium concentration
and about 5.0 mg P.L⁻¹ phosphate concentration. Notably, in the BBM medium, ammonium
levels decreased rapidly within 7 days at a concentration of 4.0 mg N.L⁻¹ whereas
in the TAP medium, it required 14 days. Conversely, phosphate concentration decline
commenced after 14 days in both algal culture environments.
