Screening of Antimetabolites
of Interest present in a Streptomycetal isolate using Analytical tools
Rath Truptimayee and Pattnaik Smaranika
Res. J. Biotech.; Vol. 20(2); 195-201;
doi: https://doi.org/10.25303/202rjbt1950201; (2025)
Abstract
Secondary metabolites are the biosynthetic products of actinomycetes cells, produced
against the competitors present the same niche. The study reports about production
of antimetabolites from soil isolated Streptomyces sp. (LMA2) co-incubated with
respective bacterial candidates (E.coli and S.aureus). The antimetabolic products
could be detected through contemporary analytical tools namely, UV-Visible spectroscopy,
FT-IR and GC-MS. The survival modality conferred by both co-incubated partners was
further substantiated by the subculture procedure. There were putative compounds
present in co-cultured broth, absorbed at 281nm and 547nm wavelengths fixed (UV-visible)
for the vancomycin and azithromycin against Gram-positive and Gram-negative bacteria
respectively.
The wave numbers (cm-1) as detected from the respective FTIR analysis, were analyzed,
referred with standard FTIR table and the putative functional groups were noted.
From the GC-MS data recorded for cell free extracts of LMA2, there were compounds
with noticeable biological activity. It was inferred that indole secreted by Streptomycetal
isolate was the major compound with absolute percentage could be a potential active
constituent to inhibit co-cultured bacterial growth.
