GC-MS analysis
and antioxidant activity of methanolic extract of Grewia asiatica L.
Khangarot Kiran, Mishra Ashmita, Singh Bharat, Bhardwaj Richa and Sharma Ram Avatar
Res. J. Biotech.; Vol. 20(1); 45-49;
doi: https://doi.org/10.25303/201rjbt045049; (2025)
Abstract
Grewia asiatica L. (Tiliaceae) is a perennial shrub, indigenously known as Phalsa
and endemic to India, Pakistan, Sri Lanka and African countries. Its fruit juice
is useful in diabetes, diabetic nephropathy and in the treatment of coronary heart
diseases. The different parts possess anti-inflammatory, antimicrobial, anticancer
and antioxidant properties. The present study was conducted to analyze the phytochemical
composition of methanolic extract of different parts of Grewia asiatica. The different
extracts prepared were evaluated for their antioxidant activity. The phytochemical
investigation was done using gas chromatography–mass spectrophotometry (GC-MS).
The antioxidant activity of methanol extract of different parts was evaluated using
DPPH and FRAP assays using ascorbic acid as standard.
GC-MS analysis reveals stigmasterol (7.47%), α-tocopherol-β-D-mannoside (4.75%),
oleic acid (3.47%) from root, 2-(hydroxymethyl)-2-nitro-1,3-propanediol (6.33%),
phytol (2.51%), neophytadiene (2.28%) from stem, n-hexadecanoic acid (12.46%),5-hydroxymethylfurfural
(4.93%), octadecanoic acid (2.14%) from flower and α -tocopherol, 2(5H)-furanone
in fruits. G. asiatica root methanolic extract showed maximum DPPH free radical-scavenging
activity (87.32 ± 0.15%) at 500 μg/ml and AEAC (557.9 ± 0.65 μM) for FRAP assay
at 1000 μg/ml concentration. Observed results reveal bioactive compounds in G. asiatica
which could be used as a source of natural antioxidants.
