Phytochemical
characterization and the therapeutic efficacy of Fraxinus americana
Jyothsna Gundlapally and Sathvika Chintalapani
Res. J. Biotech.; Vol. 20(1); 180-186;
doi: https://doi.org/10.25303/201rjbt1800186; (2025)
Abstract
Fraxinus americana, a species commonly known as Ash tree is native to Eastern and
Central North America and North Asia. This plant is known to posses a cluster of
therapeutic properties. It is being used since ages in Unani and Ayurvedic medicine.
Many infertility issues and gynecological problems can be treated by natural herbs
and their extracts, Fraxinus is one such example. Objective of the current research
is to characterize the various biochemical components present in the mother tincture
of Fraxinus americana and testing its efficacy in treating uterine fibroids based
on several biochemical and molecular assays.
Phytochemical analysis based on biochemical study was performed followed by TLC
analysis. TLC would provide an insight to the array of components present in tincture
along with the information about the suitable solvents for their separation. MTT
assay can be used to test the efficacy of the sample in controlling cell division
(anti cancer). Use of uterine fibroid related cell lines (Human Uterine Leiomyoma
cell culture) would identify its role in this condition. In addition to anti cancerous
activity by MTT, various other tests like anti inflammatory and antioxidant activity
would be performed to annotate the complete therapeutic potential of Fraxinus americana.
The study can lay a foundation for a green therapy eliminating the need for surgical
procedures in treating several uterine problems.
