Gas chromatography-mass
spectroscopy profiling of leaf extracts of Stachytarpheta urticifolia (Salisb) based
on antioxidant activities
Sreelatha Regalagadda, Kasturi Ashok Phani Kiran and Challa Murali Mohan
Res. J. Biotech.; Vol. 20(7); 91-97;
doi: https://doi.org/10.25303/207rjbt091097; (2025)
Abstract
The Stachytarpheta urticifolia is one of the widely used herbs as folk medicine
for treating various disorders by many tribal communities. Present study was to
explore the antioxidant potential of S. urticifolia leaf that was extracted with
solvents hexane, ethyl acetate and methanol. An antioxidant activity was studied
by using previous published and established methods using ABTS, hydrogen peroxide
and nitric oxide. Extracts possessing highest potential antioxidant activities were
further profiled using gas chromatography and mass spectrophotometer instrumentation.
S. utricifolia leaves have shown significant antioxidant potential with methanol
extract having inhibition concentration values IC50 = 75.1 7± 0.4 μg/ml and IC50
= 110.05 ± 7.1 μg /ml for the ABTS and hydrogen peroxide tests respectively whereas
as IC50 value was 79.01±2.02 μg/ml for the nitric oxide test.
The outcome affirms the role of S. urticifolia as a good source of antioxidant agents.
Methanolic extract of S. urticifolia leaves was further subjected for Gas Chromatography-Mass
Spectroscopic (GC–MS) analysis. In the GC-MS chromatogram, 15 compounds were obtained
with the highest percentage of peak area of 44.15 and later identified as 0,0- Diethyl
(1-formyl pentyl selenophosphate) with a retention time of 23.805 sec.
