Bacillus halodurans
RSCVS-PF21 an alkaline protease producing bacteria from poultry farm soil
Chauhan Rakesh Singh and Mishra Rahasya Mani
Res. J. Biotech.; Vol. 20(6); 98-107;
doi: https://doi.org/10.25303/206rjbt0980107; (2025)
Abstract
The aim of the study was to investigate potent alkaline protease producing indigenous
bacterial strains from alkaline soils of Vindhya region of Madhya Pradesh, India.
Accordingly, isolation and screening of alkaline protease producing alkaliphilic
bacteria was performed from different habitats of Vindhya region of Madhya Pradesh,
India. Selection and identification of promising alkaline protease producing isolates,
enzyme activity assay and partial characterization of crude alkaline protease filtrate
were done.
Many alkaline proteases producing bacterial isolates were isolated from poultry
farm soils of Vindhya region, Madhya Pradesh, India. Out of several proteolytic
isolates, most potent new bacterial strain was identified as Bacillus halodurans
RSCVS-PF21 (Genbank Accession No. MT279908) based on phenotypic, biochemical and
molecular characterizations. Crude alkaline protease was stable and effective at
high temperature. It was also highly stable in different organic solvents, so it
can be used in many industrial processes.
