Influence of Culture
conditions and Nutritional composition on the production of Antibacterial metabolite
by Pseudomonas aeruginosa VUR 102 isolated from Lake Hussain Sagar, Hyderabad, India
Konatham Samuel John and Vanga Umamaheswara Rao
Res. J. Biotech.; Vol. 20(6); 76-84;
doi: https://doi.org/10.25303/206rjbt076084; (2025)
Abstract
Microorganisms are one of the most diverse group among all the living organisms
and the metabolic products of microbes are even more diverse. Among the microbes,
bacteria are very versatile in having less generation time and ease of culturing,
strain improvement and downstream processing of the metabolite. In addition, some
of the metabolites are formed as a result of the chemical and biological stress
that bacterial isolate undergoes, which might have led to the production of metabolic
products that are chemically altered. Simple alteration even in the side chains
of active molecules has great significance in their biological activities. These
metabolic products possessing diverse biological properties, can aid in combating
emerging infectious diseases and can thereby prevent antibiotic resistance. The
present work is focussed on optimization of the bacterial isolate, Pseudomonas aeruginosa
VUR 102, which is a potent producer of antibacterial metabolite, isolated from the
lake Hussain Sagar, Hyderabad, India. The isolate, Pseudomonas aeruginosa VUR 102
exhibited an increased zone of inhibition against some selected Gram positive and
Gram negative test bacteria.
The present study concludes that the bacterial isolate Pseudomonas aeruginosa VUR
102 produces greater amounts of potent antibacterial metabolite when optimal culture
conditions and nutritional composition are provided. Optimal culture conditions
are: 120 hours of incubation period at 35oC temperature with pH 8.0 and 1% NaCl.
Nutritional composition includes the addition of glycerol and peptone as carbon
and nitrogen sources, respectively.
