Isolation and
speciation of Acinetobacter species from various clinical samples and their antibiogram
Nayaki Vidyaa S. and Mariraj Jeer
Res. J. Biotech.; Vol. 20(3); 177-180;
doi: https://doi.org/10.25303/203rjbt1770180; (2025)
Abstract
Acinetobacter species are Gram negative nonfermentative bacteria commonly present
as free living saprophytes. Acinetobacter has emerged as an important nosocomial
pathogen involved in outbreaks of hospital infections. The ubiquitous organism has
been recovered from hospital environment, from colonized or infected patients or
from staff. The emergence of multi drug resistance poses a significant challenge
in identifying and treating this organism. Retrospective study was carried on clinical
samples received from various departments. Acinetobacter isolates were identified
and speciation was done by using biochemical methods and antibiotic sensitivity
pattern of these isolates was studied.
A total of 210 Acinetobacter species were isolated from various clinical samples,
commonest being urine samples followed by sputum, blood, pus samples. The most common
species isolated were: A.baumanii (79%), A.iwoffi (17%), A.haemolyticus (2%) and
A.junni (2%). Resistant pattern to various drugs was Amoxyclauv 83.6%, Imipenam
48.3%, Piperacillintazobactum 42.6%, Amikacin 58%, Gentamicin 66%, Ceftazidime 72%,
Cefotaxime 68%, Ciprofloxacin 76%. In the present study, the prevalence of Acinetobacter
from various samples and its antimicrobial susceptibility pattern was studied. Increased
emergence of multi drug resistant strains is an important health concern as it poses
a challenge in treatment. A surveillance study is required to know about the strains
prevailing in the particular geographical locations and its antimicrobial sensitivity
pattern.
