Phytochemical
screening and Characterization using HPLC and FTIR Techniques and analysis of Antioxidant
activity of Ficus Palmata (Bedu)
Singh N., Verma R. and Aziz R.B.
Res. J. Biotech.; Vol. 20(3); 150-159;
doi: https://doi.org/10.25303/203rjbt1500159; (2025)
Abstract
From ancient times, traditional medicines have been used in Ayurveda, Siddha and
Unani. Numerous illnesses are amenable to Ficus palmata treatment belonging to the
Moraceae family. The goal of this work is to identify the chemical components through
water, petroleum ether, acetone, ethanol and methanol extracts of Ficus palmata
stems using basic phytochemical analysis, HPLC and FTIR spectroscopy. The existence
of phenols, tannins, flavonoids, alkaloids, glycosides, steroids, saponins and terpenoids
was demonstrated by the results of the phytochemical study. A C18-150 4.6 mm column,
10μ injection volume and 80:20 v/v (methanol: acetonitrile) mobile phase at 30°C
were used to conduct a reversed-phase HPLC study.
The UV detector was used to record the detection at 210 and 290 nm. FTIR analysis
of stem extracts in methanol and petroleum ether indicated the presence of aliphatic
amine compounds with significant peaks along with peaks of primary amines, alcohols,
amides, aldehydes, alkanes, alkyl halides, ethers, aromatics and alkenes. The assay
for 1,1-diphenyl-2-pycryl-hydrazyl “DPPH” and the test for hydrogen peroxide were
used to quantify the free radical scavenging activity.
