Accelerating in
vitro growth of Dendrobium chrysanthum using amino acids
Nguyen Thi Kim Anh and Tran Thanh Thang
Res. J. Biotech.; Vol. 20(5); 68-73;
doi: https://doi.org/10.25303/205rjbt68073; (2025)
Abstract
Dendrobium chrysanthum is a commercially significant orchid species, highly sought
after for both cut flowers and potted plants. However, the lengthy growth period
needed for in vitro-raised plantlets to achieve a size suitable for acclimatization
and transplantation poses a significant challenge to large-scale commercial production.
Therefore, this study aimed to assess the impact of various amino acid supplements
on in vitro growth to expedite growth rates and to diminish the duration necessary
for nursery acclimatization.
This study investigated the effects of glutamic acid, methionine, arginine and alanine
at varying concentrations (0, 25, 50, 75 and 100 mg/L). Agronomic, physiological
and biochemical parameters were consistently evaluated throughout the study. The
findings revealed that glutamic acid at a concentration of 100 mg/L notably amplified
shoot height, chlorophyll a and b levels and total soluble sugars in comparison
to the control group. These findings underscore the potential of amino acid supplementation
to optimize the in vitro culture of Dendrobium chrysanthum, thereby improving propagation
techniques for commercial production.
