An evaluation
of skin lesion treatment in mice using keratinocyte-like cell sheets consisting
of mesenchymal stem cells and a collagen scaffold
Nguyen Thanh Binh, Truong Hai Nhung, Nguyen Tuan Kiet, Gargiulo Isacco Ciro and
Huynh Duy Thao
Res. J. Biotech.; Vol. 20(5); 74-83;
doi: https://doi.org/10.25303/205rjbt74083; (2025)
Abstract
The amniotic membrane has recently garnered attention as a potential scaffold for
regenerating damaged tissues such as burns, corneal surfaces and orthopedic injuries.
Our study utilized collagen membranes derived from amniotic membranes as a scaffold
for mesenchymal stem cells obtained from umbilical cord blood to produce keratinocyte-like
cell sheets. These cell sheets were employed to treat skin loss lesions in a mouse
model. Analysis of the results indicated that the group treated with cell sheets
exhibited the most rapid tissue healing, with wound sizes healed at 83.60%, while
the untreated group showed the slowest tissue healing at 77.63%.
Histological examination revealed that utilizing cell sheets for regenerating damaged
skin tissue resulted in superior healing, with the structure of the regenerated
tissue closely resembling that of mouse skin epithelium, surpassing the outcomes
of the other two groups of mice. This research underscores the potential of keratinocyte
like cell sheets as a viable graft for autologous keratinocyte sheets or autologous
skin grafts in treating patients with skin loss lesions. Particularly beneficial
for patients lacking a sufficient supply of autologous skin grafts or requiring
immediate intervention, cell sheets can serve as a tissue graft product, offering
a viable alternative to conventional grafts.
