Effect of plant
growth regulators and culture conditions on Acrostichum aureum L. gametophytes biomass
accumulation
Ngan Nguyen-Quynh, Tan Tran-Quoc, Phuong Quach-Ngo-Diem and Anh Bui-Lan
Res. J. Biotech.; Vol. 20(5); 215-225;
doi: https://doi.org/10.25303/205rjbt2150225; (2025)
Abstract
Acrostichum aureum L. (A. aureum) is a prevalent species in mangrove areas, serving
critical functions in erosion and landslide prevention. Numerous studies have reported
on the applications and biological activities of this species such as antioxidant,
antibacterial properties, anti-inflammatory and anti-tumor activity. However, A.
aureum harvesting faces significant challenges due to the susceptibility of its
biological activities by environmental conditions such as salinity or heavy metals.
This finding focuses on the effect of in vitro conditions and plant growth regulators
to accumulate gametophyte biomass from A. aureum sterilized spores for bioactive
compound research and applications. The gametophytes were collected from sterilized
tubes, separated and placed into Petri dishes or Erlenmeyer flasks containing various
factors such as macronutrient composition, light intensity, carbohydrate content,
culture media physical states and plant growth regulators for 1 month, 2 months
and 4 months. The results show that the most effective condition for gametophyte
biomass accumulation was achieved in MS static liquid medium containing 20 g/L of
glucose placed into Erlenmeyer flask under 600 lux light conditions with 0.252 ±
0.247 g of dry weight biomass after 2 months. This suggests an effective culture
condition for biomass accumulation.
