Investigation
of antibacterial activity against some Vibrio strains of red Areca nut (Cyrtostachys
renda) ethanol extract
Phuong Vu Thi Bach, Quynh Nguyen Ngoc Nhu and Phuong Quach Ngo Diem
Res. J. Biotech.; Vol. 20(5); 111-119;
doi: https://doi.org/10.25303/205rjbt1110119; (2025)
Abstract
The Arecaceae family is known for its medicinal properties containing many natural
substances with antibacterial activity. In this study, the seeds of red Areca (Cyrtostachys
renda) belonging to the Arecaceae family were extracted from ethanol to preliminarily
survey the chemical composition and to evaluate the antibacterial ability of the
species Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus. All these
species cause acute hepatopancreatic necrosis disease (AHPNS) and white feces disease
(WFD) in shrimp. The research results showed that the red Areca nut extract contains
active ingredients such as phenol, tannin and flavonoid. The minimum inhibitory
concentration (MIC) of the red Areca nut extract against V. cholerae, V. parahaemolyticus
and V. vulnificus was 12.50 mg/ml, 3.13 mg/ml and 6.25 mg/ml respectively and the
minimum bactericidal concentration (MBC) was 200.00 mg/ml, 16.67 mg/ml and 10.42
mg/ml respectively.
The results of the survey on the increase of V. cholerae, V. parahaemolyticus and
V. vulnificus in TSA medium supplemented with red Areca nut ethanol extract over
time (Time-kill curve assay) showed that red Areca nut extract had the best resistance
to V. parahaemolyticus strain and completely killed V. parahaemolyticus at MIC concentration
(3.13 mg/ml) after 2 hours of culture in living medium. Next, at 0.25MIC concentration
(1.56 mg/ml), red Areca nut extract could completely kill V. vulnificus after 4
hours of culture in liquid medium. Finally, at MIC concentration (12.50 mg/ml),
red Areca nut extract has the ability to completely destroy V.cholerae after 2 hours
of culture in liquid medium.
