In vitro Propagation
and Variation of Antioxidant and Docking Properties of Bacopa monnieri (L.) Wettst.
a Threatened Medicinal Plant
Govindarasu Sekar and Shanmugasundharam Murugesh
Res. J. Biotech.; Vol. 20(11); 142-149;
doi: https://doi.org/10.25303/2011rjbt1420149; (2025)
Abstract
Bacopa monnieri (L.) Wettsts Ayurveda herb has anti-cancer, anti-ulcer, antispasmodic,
anti-asthmatic, neuro-tonic, immuno-modulator, adaptive, tranquillizing, memory
and learning improving, cerebral activator and therapeutic value. It is most well-known
for its nootropic properties. Through the antioxidant and molecular docking stimulation
of several shoots, Bacopa monnieri(L.) Wettst is analyzed. In this investigation,
methanol extracts exhibited the strongest MeoH free radical scavenging capabilities,
with the antioxidant activity increasing noticeably and regenerated in vitro in
the current study. The molecular docking study evaluated the binding affinities
of various compounds with four protein targets. The docking scores (in kcal/mol)
indicate the strength of interaction where a lower (more negative) value suggests
stronger binding affinity. Leaf explants were used. Explants that had been surface-sterilized
were aseptically cultivated on MS media that included varying amounts of plant growth
regulators.
The leaf responded most favorably to shoot regeneration and subsequent plant development
on MS. After six weeks of culture, the leaf explants produced an average of 12.94
± 2.81 shoots/explants in this combination. The optimal medium for root induction
was determined to be MS half-strength supplemented with 0.5 mg/L IBA. Thus, MeoH
has great potential as a natural remedy for a number of inflammatory, oxidative
and infectious disorders. For additional research, screening of its active components
is required. The in vitro grown rooted plantlets were placed in plastic pots to
garden in a 50:50 soil vermiculite mixture. After that, the acclimatized plants
were successfully moved into the soil.
