Influence of cultural
conditions on enhancing chitinase produced by Paenibacillus polymyxa M6 strain isolated
from soil at Thanh Tra pomelo (Citrus grandis (L.) Osbeck) farm in Vietnam
Hoang Ha My A., Trai Vo Thi Ngoc, Dung Tran Quoc and Hung Nguyen Bao
Res. J. Biotech.; Vol. 20(11); 42-48;
doi: https://doi.org/10.25303/2011rjbt042048; (2025)
Abstract
In this study, we investigated the culture conditions to optimize the production
of extracellular chitinase from a Paenibacillus sp. strain isolated from rhizosphere
of pomelo orchards affected by gummosis. The enzyme exhibited maximum activity (56.68
U/ml) under conditions where soy flour served as the nitrogen source. Colloidal
chitin concentration was maintained at 0.3%, initial pH was set to 3 and static
culture was maintained at 30oC for 72 hours, with the inoculum continuing at 5%
(v/v).
The results demonstrate the strain's potential for chitinase production, suggesting
its suitability for further development and application in products aimed at preventing
gum disease in Thanh Tra pomelo.
