Isolation and
phylogenetic characterisation of an antimicrobially active bacterial strain from
the Telescopium telescopium
Yedla Suneetha, Upmaka Yamini and Gudivada Mani
Res. J. Biotech.; Vol. 20(11); 10-20;
doi: https://doi.org/10.25303/2011rjbt010020; (2025)
Abstract
Antimicrobial resistance is a global concern that diminishes the options for treating
diseases caused by microbes. The WHO global report indicates a rise in mortality
from infectious diseases attributable to antimicrobial resistance, leading to a
global economic loss of up to 100 trillion USD by 2050. Consequently, the identification
of novel antibacterial agents having clinical relevance is imperative. The diversity
of marine ecosystems has led to the discovery of a wide range of metabolites with
bioactive qualities. Conversely, numerous bioactive compounds linked to marine invertebrates
are synthesised by their symbiotic microbes. Thus, the current work focused on the
identification of bacterial strains from Telescopium telescopium exhibiting antimicrobial
potential. This investigation isolated 10 distinct bacterial colonies, designated
as ZTS-01 to ZTS-10. Among the 10 isolates, ZTS-08 showed superior antimicrobial
activity against the examined bacterial and fungus species except C. albicans.
The PCR-amplified 16s rRNA gene of ZTS-08 comprises of 1500 bp in length and its
G+C proportion is 58.46%. The multiple sequence alignments reveal variance, conserved
sites and parsimony sites of 0.88, 0.06 and 0.021 respectively, with an overall
mean distance of 0.01. According to molecular phylogenetics and morphological analyses,
the bacterial isolate ZTS-08 is a member of the N. dassonvillei species.
