Screening and
Evaluation of Antioxidant and Cytotoxic Potential of Herbal Mixture with Annona
muricata and Guilandina bonduc against Cancer lines (A 549, SiHa, HeLa) -A Systematic
Study
Koshy Feba Sara, Karpakavalli M., Chandrasekaran Rajkuberan, Ranjithkumar D. and
Sangilimuthu Alagar Yadav
Res. J. Biotech.; Vol. 20(11); 264-271;
doi: https://doi.org/10.25303/2011rjbt2640271; (2025)
Abstract
Polyherbal formulations are widely utilized in ethnomedicine due to their synergistic
effects and broad-spectrum bioactivity. This study aimed to evaluate the antioxidant
properties, cytotoxic potential against cancer cell lines and to perform GC-MS profiling
of a polyherbal mixture derived from Annona muricata leaves and Guilandina bonduc
seeds. Ethanolic extracts of both plants were prepared using Soxhlet extraction
and combined in a 1:1 ratio. Standardization involved phytochemical screening, determination
of total phenolic content (TPC), total flavonoid content (TFC) and assessing antioxidant
activity using DPPH and ABTS assays and cytotoxicity screening.
Phytochemical analysis confirmed the presence of alkaloids, flavonoids, phenolics,
tannins and terpenoids. GC-MS analysis identified active bioactive constituents
including 9,12-octadecadienoic acid, phytol, caryophyllene and squalene. Herbal
mixture shows low cytotoxicity to normal cells and moderate cytotoxicity to cancer
cells, especially A549 (lung cancer). The herbal mixture demonstrated significant
antioxidant potential and moderate cytotoxicity towards cancer cell lines (A 549
and SiHa), supporting its traditional use and paving the way for further pharmacological
investigations.
