Comparative studies
on mitigation of the deteriorative effect of carbon tetrachloride in rates: Effect
of Terminalia belerica and Gallic acid – Lipoic acid combination
Jadon Anjana, Singh Kuldeep, Singh Raman, Srivastava Sadhana and Shukla Sangeeta
Res. J. Biotech.; Vol. 20(10); 262-271;
doi: https://doi.org/10.25303/2010rjbt2620271; (2025)
Abstract
Terminalia belerica fruit extract (TB 400 mg / kg, po for five days) and its active
principle: gallic acid (GA 200 mg / kg, po for five days) were studied against carbon
tetrachloride (CCl4 0.15 ml / kg, ip for 21 days) induced liver injury in rats.
Toxicants increased blood sugar and serum protein (P 0.05). Induced liver necrosis
increased liver-maker enzyme activity (AST, ALT, SALP and LDH), oxidative stress
parameters and histopathology including necrosis, hepatocyte degeneration and inflammatory
cell infiltration. CCl4 increased albumin and bilirubin. Carbon tetrachloride intoxication
altered urea and creatinine. GA and LA therapy was effective, with values close
to control and comparable to silymarin. At 0.15 ml/kg i.p. for 21 days, carbon tetrachloride
increased hepatic triglycerides and serum cholesterol (P 0.05).
Hepatic lipid peroxidation increased. CCl4 decreased reduced glutathione. CCl4 harmed
succinic dehydrogenase, adenosine triphosphatase, acid and alkaline phosphatases.
GA and LA reduced oxidative damage from CCl4 gallic acid and lipoic acid synergized.
GA and LA acid improved liver function and oxidative stress markers. The current
findings suggest that five-day GA and LA treatment could prevent liver injury in
rats.
