Evaluation of
Bioremediation Competence of Indigenous Bacterial Strain Brevibacillus panacihumi
KUESCCHK-5 isolated from Textile Effluent and its Effects on Vigna mungo
Chaithra C. and Kousar Hina
Res. J. Biotech.; Vol. 20(10); 285-295;
doi: https://doi.org/10.25303/2010rjbt2850295; (2025)
Abstract
The textile industry generates highly contaminated wastewater containing harmful
substances, posing significant environmental hazards. Bioremediation using natural
organisms offers an effective treatment solution. This study involved the characterization
of physico-chemical parameters of textile wastewater, followed by treatment using
indigenous bacterial strain isolated from the effluent. The bacterial strain isolated
from textile effluent identified as Brevibacillus panacihumi KUESCCHK-5 via 16S
rRNA gene sequencing, was evaluated for its bioremediation potential under static
conditions over five days. The strain achieved a decolorization rate of 99.38%-100%
under optimal conditions (40°C, pH 9.5, peptone as a nitrogen source, glucose as
a carbon source with a 4:1 carbon-to-nitrogen ratio). The treatment significantly
reduced pollution parameters: BOD by 94.16%-99.11%, COD by 89.24%-98.83%, electrical
conductivity by 79.06%-88.97%, fluoride by 89.08%-97.81%, phosphate by 91.38%-97.94%,
turbidity by 89.76%-97.44% and sodium by 89.88%-97.65% at different effluent concentrations
(Raw, 75%, 50% and 25% concentrations).
ANOVA analysis indicated significant differences in pollutant levels across different
effluent concentrations (p = 0.0436) and t-tests showed that the removal efficiency
of physico-chemical parameters was significant at the 5% level but not at the 1%
level. Additionally, heavy metals were reduced to below detectable limits and the
treated effluent was non-toxic to Vigna mungo. These results indicate that Brevibacillus
panacihumi is a promising, eco-friendly and cost-effective agent for treating textile
effluent.
