Isolation and
characterization of a bioactive compound with pharmacological potential from Salacia
oblonga endophytome
Sushma T., Roopa G., Pushpalatha H.G., Geetha N. and Madhusudhan M.C.
Res. J. Biotech.; Vol. 20(10); 311-318;
doi: https://doi.org/10.25303/2010rjbt3110318; (2025)
Abstract
The current work emphasizes the isolation and characterization of the bioactive
compound from an endophytic fungus isolated from medicinal plant Salacia oblonga.
Antimicrobial, antioxidant and antidiabetic activities of the active fraction were
confirmed using standard methods. The extracts showed significant antibacterial
activity against some pathogenic bacteria and were also positive to in vitro antidiabetic
assays. The IC50 values obtained in the antioxidant assays (34.56 μg for SOEF fraction
and 19.65 μg for ascorbic acid in the case of DPPH assay and 38.34 μg for SOEF fraction
and 19.65 μg for quercetin in the case of ABTS assay) were positive and comparable
to the standards used.
The purified active fraction was identified and characterized using LC-MS, NMR,
FTIR, Homonuclear correlation spectroscopy sequence (COSY) and Heteronuclear Multiple
Bond Coherence (HMBC). From the studies, the compound obtained in the active fraction
was found to be 3-(4-hydroxyphenyl) propionic acid, a potent antioxidant compound
that has several applications in food and pharma industries.
