Genetic diversity
of the ornamental plant Anthurium andraeanum in Vietnam
Sen Thi Trinh, Nhi Thi Hoang Ho, Han Ngoc Ho and Hai Thi Hong Truong
Res. J. Biotech.; Vol. 20(9); 159-165;
doi: https://doi.org/10.25303/209rjbt1590165; (2025)
Abstract
Anthurium sp. is an imported ornamental plant with high economic value in Vietnam.
Consumer preferences and demand depend greatly on the colour of the spathes, so
understanding the genetic diversity of varieties is necessary to create novel flowers.
In this work, we aimed to characterise genetic diversity among Anthurium accessions
collected from four provinces in Vietnam (Ha Noi, Thua Thien Hue, Lam Dong and Can
Tho). DNA of eighteen Anthurium accessions was extracted using the cetyl trimethyl
ammonium bromide (CTAB) method. Then, genomic DNA was amplified with matK and rbcL
primers in PCR and sequencing to determine sequence parameters using DNAsp v6 and
MEGA11 software. Three phylogenetic trees including the Neighborjoining (NJ) tree,
Maximum likelihood (ML) tree and Bayesian inference (BI) tree were created to separate
Anthurium accessions. In addition, ten morphological traits were also described
and were cluster-analysed (UPGMA) using MVSP v3.22 software.
The matK yielded the highest diversity information with 54 variable (polymorphic)
sites, 52 parsimony informative sites, 6 Insertion-Deletion (InDel) sites and nucleotide
diversity at 0.01195. Besides, intraspecific distances ranged from 0.0000 to 0.0058
and interspecific genetic distances ranged from 0.0000 to 0.0645. Furthermore, phylogenetic
tree analysis using DNA barcoding results and morphological description data classified
accessions into two groups corresponding to the Anthurium and Caladium species.
In particular, HUIB_AA06 was distinct from the remaining accessions.
