Application of
ISSR markers to analyze the genetic diversity of the May nuoc mo species (Calamus
sp.) in Quang Nam province, Central Vietnam
Hieu Huynh Kim, Loi Nguyen Van, Long Dang Thanh and Minh Nguyen Van
Res. J. Biotech.; Vol. 21(2); 1-9;
doi: https://doi.org/10.25303/212rjbt0109; (2026)
Abstract
The May nuoc mo species (Calamus sp.) is used as a raw material for the production
of handicrafts and household wares. It is a species with high economic value that
contributes to improving the livelihoods of planters. In this study, a total of
23 Calamus sp. individuals collected from two districts, Dong Giang and Nam Giang,
in Quang Nam province were evaluated for genetic diversity using the molecular marker
ISSR (Inter Simple Sequence Repeat). Out of 113 DNA bands, 91 were polymorphic DNA
bands and 22 were monomorphic from fifteen ISSR primers. No private band DNA differences
were detected among the studied Calamus sp. individuals.
The proportion of polymorphic DNA bands obtained from each primer ranged from 50
to 100% (mean = 79.918%). The level of genetic similarity between individuals in
Calamus sp. populations ranges from 67% to 96%, indicating a high degree of genetic
variation among the Calamus sp. individuals studied in different localities. The
genetic tree showed that different Calamus sp. individuals in the same locality
had high genetic similarity and were distributed on the same evolutionary branch.
It is proposed to use Calamus sp. populations distributed in different localities
in this study as seedlings to maintain genetic resources and develop planted forests
according to forestry programs. This would contribute to improving production value
and enhancing people's livelihoods.
