Identification
and validation of molecular markers for rust resistance in wheat genotypes for marker
assisted breeding in India
Singh Prashant, Mishra Vinod Kumar, Atri Neelam, Ashutosh, Kumar Monu and Yadav
Manoj Kumar
Res. J. Biotech.; Vol. 21(2); 264-270;
doi: https://doi.org/10.25303/212rjbt2640270; (2026)
Abstract
Rust disease is caused by fungus that wreaks havocon aerial parts of the wheat crop
and has been identified as one of the most destructive diseases in wheat in almost
all parts of India. This disease may potentially reduce wheat yields up to 20% globally.
Validation of linked marker genes for various biotic and abiotic characters suggested
that the linked genes can be used in marker assisted breeding programs. In the present
experiment, phenotypic screening against different pathotypes of leaf and stripe
rust at Shimla, Himachal Pradesh was carried out and it has been found that FLW30
shows resistance against nine rust pathotypes i.e. four pathotypes for stripe rust
and five pathotypes for leaf rust.
Three resistance genes including leaf rust resistance (Lr28) and stripe rust resistance
(Yr9 and Yr15) were validated in different donor parents i.e. FLW29, FLW30 and PBW343
and two Gpc-B1 improved lines of HUW234 and HUW468 (iHUW234 and iHUW468) as recipient
parents. On validation, it has been found that FLW30 contained all three rust resistance
genes, whereas PBW 343 possessed Lr28 and Yr15 genes and FLW29 has only Yr9gene
in their background. Therefore, the identified wheat genotypes could be used as
potential donor for leaf and stripe rust resistance in marker-assisted breeding.
