Research Journal of Biotechnology

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Isolation and Characterization of Phosphate Solubilizing Microorganisms from Saccharum officinarum Rhizosphere

Tomer Supriya, Parveen Shaiba and Khati Priyanka

Res. J. Biotech.; Vol. 21(2); 178-184; doi: https://doi.org/10.25303/212rjbt1780184; (2026)

Abstract
Deficits in phosphate can hinder plant growth and development because it is essential to all aspects of plant growth and development. The present study focused on isolation and evaluation of soil bacterial isolated from 4 different locations. The 21 isolates recovered were subjected to phosphate solubilization and siderophore productions assay. As per the results 9 bacterial isolated (V1, V2, V3, V11, V12, V13, CV1, CV2 and CV11) were found best phosphate solubilizers on the basis of qualitative phosphate assay. Out of 9 screened bacterial isolated only 3 were best suited for siderophore production (V2, V11 and V13). The pqq genes responsible for phosphate solubilization were also checked through amplification and as per the observation, 5 bacterial isolates showed positive amplification (V1, V2, V3, V11, V13 and CV11).

The identified isolates present potential phosphate solubilizing ability of bacterial isolates which can further be applied in the field of biofertilizer development and commercialization.