DMSO-Mediated
Induction of Lacthydrazide in Streptomyces sp. VITGV100 and its Molecular Docking
Evaluation
Moon Madhuri Mukindrao and Christopher John Godwin
Res. J. Biotech.; Vol. 21(1); 309-319;
doi: https://doi.org/10.25303/211rjbt3090319; (2026)
Abstract
Streptomyces species are renowned for their ability to produce bioactive secondary
metabolites, many of which act as essential antimicrobials against infectious diseases.
As multidrug-resistant (MDR) microorganisms pose an increasing global threat, the
need for alternative antimicrobial agents has become urgent. In this study, Streptomyces
sp. VITGV100 was cultured under static conditions with 0.5% dimethyl sulfoxide (DMSO)
as an elicitor to boost secondary metabolite production. The bioactive compounds
were extracted using ethyl acetate and were analyzed by liquid chromatography-mass
spectrometry (LC-MS). The separation of the active ingredients was performed using
thin layer chromatography (TLC). Comparative metabolic profiling and molecular docking
studies were conducted to investigate the structural and functional characteristics
of these compounds.
The results identified lacthydrazide as a novel antimicrobial agent with notable
inhibitory effects against Escherichia coli, Staphylococcus aureus, Bacillus subtilis
and Pseudomonas aeruginosa. Fourier Transform Infrared Spectroscopy (FT-IR) was
used to identify functional groups, aiding in structural characterization complemented
by bioinformatic analyses. Molecular docking against antimicrobial target proteins
(PDB IDs: 1KZN, 1GSK, 4RLC and 4URM) showed strong binding affinities and significant
protein-ligand interactions, supporting the compound’s potential as an effective
antimicrobial. This research offers a detailed analysis of lacthydrazide, a metabolite
produced by Streptomyces sp. VITGV100 and highlights the importance of DMSO in activating
cryptic biosynthetic pathways. These findings advance the ongoing search for new
antimicrobial drugs from Streptomyces species.
