Isolation and
identification of microbial strains producing antineoplastic agent L-asparaginase
Shukla Aparna, Vishal Kunwar and Yadav Pramod K.
Res. J. Biotech.; Vol. 21(1); 172-176;
doi: https://doi.org/10.25303/211rjbt1720176; (2026)
Abstract
L-asparaginase (3.5.1.1) is an amidohydrolase enzyme that provides a catalytic site
for converting asparagine into ammonia and aspartate. It is used to treat various
types of cancer especially acute lymphoblastic leukemia. Our study aims to screen
and identify indigenous fungal strains that produce asparaginase enzymes using plate
assay method. In this study we isolated and screened four fungal strains. Out of
four strains, one fungal strain was found to have maximum asparaginase production.
Further, the fungal strain was subjected to liquid culture media, the maximum production
of the enzyme was found on eight days of inoculation.
