Spectrophotometric
Determination of Biotin in Drug Samples using Ligand Substitution Reaction
Srivastava A., Srivastava N. and Singh R.
Res. J. Chem. Environ.; Vol. 29(4); 7-14;
doi: https://doi.org/10.25303/294rjce07014; (2025)
Abstract
A spectrophotometric approach that is straightforward, efficient, highly sensitive
and precise has been devised to quantify biotin (BTN) in both its pure state and
pharmaceutical samples. The methodology relies on the inhibitory approach of BTN
on the Hg(II) promoted ligand substitution (LS) reaction involving pyrazine (PYZ)
and hexacyanoferrate(II). The process entails replacing cyanide in [Fe(CN)6]4- with
PYZ, triggering the development of a complex [Fe(CN)5PYZ]3-. The complex demonstrates
a significant absorption level at a specific wavelength of 370 nm. The established
limit of detection for BTN is 0.075μg mL−1.
Recovery experiments were conducted to confirm the precision and accuracy of BTN
quantification. The suggested approach has been effectively utilized for the examination
of BTN in pristine samples and various medications, demonstrating remarkable levels
of precision and accuracy. The outcomes showed good agreement when compared to the
findings of the official analytical method. The excipients typically employed in
medicines do not exhibit any interference with the suggested methodology.
