Multiple Objective
Optimization of High Nitrogen content Biofertilizer Production using Mushroom waste
Syarifudin Baharudin Amir, Norazwina Zainol and Hazwani Aziz Nor
Res. J. Chem. Environ.; Vol. 29(8); 1-10;
doi: https://doi.org/10.25303/298rjce01010; (2025)
Abstract
The study aimed to optimize nitrogen content in biofertilizer production using mushroom
waste. Five factors were analyzed using two-level full factorial design (TLFD) including
mushroom waste content, reaction time for Bokashi fermentation, agitation of mushroom
waste, size of mushroom waste and drying method. The nitrogen content was measured
using a HACH Spectrophotometer. The factors were further optimized using a central
composite design (CCD) through response surface methodology. The parameters studied
included drying temperature and mushroom waste content.
Analysis of variance (ANOVA) showed that the best conditions for producing high
nitrogen biofertilizer were a drying temperature of 104 ℃, 70% mushroom waste content,
uncut mushroom waste size, a Bokashi fermentation reaction time of 10 days with
agitation and nitrogen content of up to 1624 mg/L. The suggested optimum condition
for producing high nitrogen biofertilizer was a temperature of 80°C and 70% mushroom
waste content, yielding the highest nitrogen content of 1305mg/L. This research
suggests mushroom waste as a new alternative for biofertilizer production, adding
value-added natural products.
