Green synthesis:
silver nanoparticles from microbial extract for biogas generation
Pathare Aishwayra, Desai Hindavi, Sarode Sneha, Nibandhe Shalmali and Jamkhedkar
Suruchi
Res. J. Chem. Environ.; Vol. 29(1); 61-66;
doi: https://doi.org/10.25303/291rjce061066; (2025)
Abstract
The primary objective of this study is the green synthesis of silver nanoparticles
using the active extract obtained from microorganisms. The microorganism, a new
strain of Bacillus hunanensis, was chosen from landfill leachate of Durgadi Fort
dumping ground near Kalyan and soil near the decomposer machine of University of
Mumbai, Vidyanagari campus. The new strain of Bacillus hunanensis (Accession Number:
MK951982) culture was centrifuged and was sonicated for 10 min. The culture was
once again centrifuged after being sonicated. The supernatant was decanted and distilled
water was used to wash the precipitate.
The process of producing silver nanoparticles involved reacting a standard silver
salt solution with an aqueous bio-reducing solution obtained from the microorganism.
The reaction progress was monitored by analytical techniques. The nanoparticles
obtained were characterized by the FTIR, TEM and SEM-EDS. Further the nanoparticles
produced were used in the digester for biogas emission.
