Antioxidant and
antibacterial Potential of Phytochemicals extracted from Ehretia laevis–an in vitro
and in silico analysis
Vivek K., Abdhul K., Mahendran D., Poonkothai M. and Saravanan K.
Res. J. Chem. Environ.; Vol. 29(6); 38-50;
doi: https://doi.org/10.25303/296rjce038050; (2025)
Abstract
The present study aims to identify antibacterial and antioxidant phytochemical drug
from Ehretia laevis. This study reports detailed phytochemical and biological investigations
of newly identified compounds from E.laevis. Soxhlet extraction, qualitative and
quantitative phytochemical, antibacterial assay and antioxidant property of crude
extract were used as preliminary screening. The secondary screening docking was
performed followed by GCMS. Data suggest that the phytochemical analysis reveals
presence of phenols, tannins, saponins, glycosides, flavonoids and alkaloids in
ethyl acetate extraction. Protein, flavonoids saponins, steroids, terpenoids and
Alkaloids in chloroform were identified. High amounts of phenol, flavonoid and alkaloid
were recorded in ethylacetate extract than chloroform extract. MIC was best at EA
extract recorded between 12.5 to 25 μg. GCMS reveals presence of 20-30 compounds
with some novel phytochemicals like amyrin, 6-bromo-5-oxo-3-methylhexan, 1,3-heptadiene,
7-azido-3-(1-ethoxyethoxy)-, (e)-(.+-.)-, 1H-furo[3,4-c]pyrrole-4-carboxylic acid,
6-(2-furanyl)hexahydro-1,3-dioxo-4-phenyl-, methyl ester etc.
In silico docking of amyrin and 1H-furo[3,4-c]pyrrole-4-carboxylic acid reveals
that both compounds are capable to form strong hydrogen bonds comparatively equal
to standard drug and thus inhibit tRNA synthetases and nitric oxide synthase protein.
The newly isolated triterpenoid amyrin and carboxlic acid 1H furan derivative may
serve as potential leads for the development of novel anti-iNOS agents and antibacterial
compound and have drug likeliness properties.
