Isolation and
selection of highly degrading chlorpyrifos bacillus spp. from tea plantation soils
Pham Quoc Cuong, Tran Minh Bao, Le Đuc Trung, Nguyen Thanh Lam and Nguyen Duong
Tam Anh
Res. J. Chem. Environ.; Vol. 29(5); 1-7;
doi: https://doi.org/10.25303/295rjce0107; (2025)
Abstract
Bacillus is a genus widely used in agriculture as well as in environmental remediation.
This study aimed to isolate and select Bacillus strains with high chlorpyrifos (CP)
degradation ability from five tea growing areas in Da M'Bri, Bao Loc city, Lam Dong
province, Vietnam where pesticides are commonly used in cultivation. Fifty-seven
bacterial strains were isolated from 25 tea growing soil samples using minimal salt
medium (MSM) agar containing 300 μg/ml chlorpyrifos as the sole carbon source. These
bacterial isolates were evaluated for their ability to grow in MSM agar with increasing
CP concentrations from 100 to 500 μg/ml and their CP degradation ability was quantified.
Three strains, designated as L1D3.3, L4D2.2 and L4D1.1, exhibited the highest chlorpyrifos
degradation efficiencies of 88.04%, 88.78% and 91.74% respectively. These strains
were identified and characterized as Bacillus megaterium (L1D3.3), Bacillus aerius
(L4D2.2) and Bacillus altitudinis (L4D1.1) followed by Bergey’s manual and 16S rRNA
full length gene sequence analysis.
